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2 "Glucose-stimulated insulin secretion"
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Original Article
Hexane Extract of Orthosiphon stamineus Induces Insulin Expression and Prevents Glucotoxicity in INS-1 Cells
Hae-Jung Lee, Yoon-Jung Choi, So-Young Park, Jong-Yeon Kim, Kyu-Chang Won, Jong-Keun Son, Yong-Woon Kim
Diabetes Metab J. 2015;39(1):51-58.   Published online February 16, 2015
DOI: https://doi.org/10.4093/dmj.2015.39.1.51
  • 4,530 View
  • 62 Download
  • 10 Web of Science
  • 8 Crossref
AbstractAbstract PDFPubReader   
Background

Hyperglycemia, a characteristic feature of diabetes, induces glucotoxicity in pancreatic β-cells, resulting in further impairment of insulin secretion and worsening glycemic control. Thus, preservation of insulin secretory capacity is essential for the management of type 2 diabetes. In this study, we evaluated the ability of an Orthosiphon stamineus (OS) extract to prevent glucotoxicity in insulin-producing cells.

Methods

We measured insulin mRNA expression and glucose-stimulated insulin secretion (GSIS) in OS-treated INS-1 cells after exposure to a high glucose (HG; 30 mM) concentration.

Results

The hexane extract of OS elevated mRNA expression of insulin as well as pancreatic and duodenal homeobox-1 of INS-1 cells in a dose-dependent manner. The hexane OS extract also increased the levels of phosphorylated phosphatidylinositol 3-kinase (PI3K) in a concentration-dependent manner. Additionally, Akt phosphorylation was elevated by treatment with 100 and 200 µmol of the hexane OS extract. Three days of HG exposure suppressed insulin mRNA expression and GSIS; these expressions were restored by treatment with the hexane OS extract. HG elevated peroxide levels in the INS-1 cells. These levels were unaffected by OS treatment under both normal and hyperglycemic conditions.

Conclusion

Our results suggested that the hexane extract of OS elevates insulin mRNA expression and prevents glucotoxicity induced by a 3-day treatment with HG. This was associated with the activation of PI-3K and Akt.

Citations

Citations to this article as recorded by  
  • An Updated Review of Ethnobotany, Ethnopharmacology, Phytochemistry and Pharmacological Activities of Orthosiphon stamineus Benth
    Anandarajagopal Kalusalingam, Dania Najiha Hasnu, Abdullah Khan, Ching Siang Tan, Bama Menon, Venkateshan Narayanan, Khang Wen Goh, Asmuni Mohd Ikmal, Noraini Talip, Poonguzhali Subramanian, Long Chiau Ming
    Malaysian Applied Biology.2024; 53(1): 1.     CrossRef
  • Scopoletin protects INS-1 pancreatic β cells from glucotoxicity by reducing oxidative stress and apoptosis
    Jae Eun Park, Ji Sook Han
    Toxicology in Vitro.2023; 93: 105665.     CrossRef
  • A Systematic Review of Orthosiphon stamineus Benth. in the Treatment of Diabetes and Its Complications
    Qirou Wang, Jia Wang, Nannan Li, Junyu Liu, Jingna Zhou, Pengwei Zhuang, Haixia Chen
    Molecules.2022; 27(2): 444.     CrossRef
  • Comprehensive chemical and metabolic profiling of anti‐hyperglycemic active fraction from Clerodendranthi Spicati Herba
    Yun Luo, Yue Liu, Quan Wen, Yulin Feng, Ting Tan
    Journal of Separation Science.2021; 44(9): 1805.     CrossRef
  • Short-Term Protocols to Obtain Insulin-Producing Cells from Rat Adipose Tissue: Signaling Pathways and In Vivo Effect
    Krista Minéia Wartchow, Letícia Rodrigues, Lucas Zingano Suardi, Barbara Carolina Federhen, Nicholas Guerini Selistre, Carlos-Alberto Gonçalves, Patrícia Sesterheim
    International Journal of Molecular Sciences.2019; 20(10): 2458.     CrossRef
  • Understanding glycaemic control and current approaches for screening antidiabetic natural products from evidence-based medicinal plants
    Chintha Lankatillake, Tien Huynh, Daniel A. Dias
    Plant Methods.2019;[Epub]     CrossRef
  • 50% Ethanol extract of Orthosiphon stamineus modulates genotoxicity and clastogenicity induced by mitomycin C
    Dhamraa Waleed Al-dualimi, Aman Shah Abdul Majid, Sarah Furqan Faisal Al-Shimary, Amal Aziz Al-Saadi, Raghdaa Al Zarzour, Muhammad Asif, Chern Ein Oon, Amin Malik Shah Abdul Majid
    Drug and Chemical Toxicology.2018; 41(1): 82.     CrossRef
  • Can Tea Extracts Exert a Protective Effect Against Diabetes by Reducing Oxidative Stress and Decreasing Glucotoxicity in Pancreatic β-Cells?
    Heeyoung Chae, Patrick Gilon
    Diabetes & Metabolism Journal.2015; 39(1): 27.     CrossRef
Review
Fuel-Stimulated Insulin Secretion Depends upon Mitochondria Activation and the Integration of Mitochondrial and Cytosolic Substrate Cycles
Gary W. Cline
Diabetes Metab J. 2011;35(5):458-465.   Published online October 31, 2011
DOI: https://doi.org/10.4093/dmj.2011.35.5.458
  • 65,535 View
  • 38 Download
  • 9 Crossref
AbstractAbstract PDFPubReader   

The pancreatic islet β-cell is uniquely specialized to couple its metabolism and rates of insulin secretion with the levels of circulating nutrient fuels, with the mitochondrial playing a central regulatory role in this process. In the β-cell, mitochondrial activation generates an integrated signal reflecting rates of oxidativephosphorylation, Kreb's cycle flux, and anaplerosis that ultimately determines the rate of insulin exocytosis. Mitochondrial activation can be regulated by proton leak and mediated by UCP2, and by alkalinization to utilize the pH gradient to drive substrate and ion transport. Converging lines of evidence support the hypothesis that substrate cycles driven by rates of Kreb's cycle flux and by anaplerosis play an integral role in coupling responsive changes in mitochondrial metabolism with insulin secretion. The components and mechanisms that account for the integrated signal of ATP production, substrate cycling, the regulation of cellular redox state, and the production of other secondary signaling intermediates are operative in both rodent and human islet β-cells.

Citations

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  • Nicotinamide Adenine Dinucleotide: The Redox Sensor in Aging-Related Disorders
    Giovanna Schiuma, Djidjell Lara, James Clement, Marco Narducci, Roberta Rizzo
    Antioxidants & Redox Signaling.2024;[Epub]     CrossRef
  • ROCK1 regulates insulin secretion from β-cells
    Byung-Jun Sung, Sung-Bin Lim, Won-Mo Yang, Jae Hyeon Kim, Rohit N. Kulkarni, Young-Bum Kim, Moon-Kyu Lee
    Molecular Metabolism.2022; 66: 101625.     CrossRef
  • Hyperglycemic Stress and Carbon Stress in Diabetic Glucotoxicity
    Xiaoting Luo, Jinzi Wu, Siqun Jing, Liang-Jun Yan
    Aging and disease.2016; 7(1): 90.     CrossRef
  • Characterization of Phospholipids in Insulin Secretory Granules and Mitochondria in Pancreatic Beta Cells and Their Changes with Glucose Stimulation
    Michael J. MacDonald, Lacmbouh Ade, James M. Ntambi, Israr-Ul H. Ansari, Scott W. Stoker
    Journal of Biological Chemistry.2015; 290(17): 11075.     CrossRef
  • Roles of Pyruvate, NADH, and Mitochondrial Complex I in Redox Balance and Imbalance inβCell Function and Dysfunction
    Xiaoting Luo, Rongrong Li, Liang-Jun Yan
    Journal of Diabetes Research.2015; 2015: 1.     CrossRef
  • Peroxisome Proliferator-activated Receptor γ (PPARγ) and Its Target Genes Are Downstream Effectors of FoxO1 Protein in Islet β-Cells
    Dhananjay Gupta, Averi A. Leahy, Navjot Monga, Mina Peshavaria, Thomas L. Jetton, Jack L. Leahy
    Journal of Biological Chemistry.2013; 288(35): 25440.     CrossRef
  • Desnutrin/ATGL Activates PPARδ to Promote Mitochondrial Function for Insulin Secretion in Islet β Cells
    Tianyi Tang, Marcia J. Abbott, Maryam Ahmadian, Andressa B. Lopes, Yuhui Wang, Hei Sook Sul
    Cell Metabolism.2013; 18(6): 883.     CrossRef
  • Potent humanin analog increases glucose‐stimulated insulin secretion through enhanced metabolism in the β cell
    Regina Kuliawat, Laura Klein, Zhenwei Gong, Marianna Nicoletta‐Gentile, Anjana Nemkal, Lingguang Cui, Claire Bastie, Kai Su, Derek Huffman, Manju Surana, Nir Barzilai, Norman Fleischer, Radhika Muzumdar
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Diabetes Metab J : Diabetes & Metabolism Journal