Diabetes & Metabolism Journal

Original Article

Basic Research
Glucagon-Like Peptide Receptor Agonist Inhibits Angiotensin II-Induced Proliferation and Migration in Vascular Smooth Muscle Cells and Ameliorates Phosphate-Induced Vascular Smooth Muscle Cells Calcification: Jinmi Lee, Seok-Woo Hong, Min-Jeong Kim, Sun Joon Moon, Hyemi Kwon, Se Eun Park, Eun-Jung Rhee, Won-Young Lee; Diabetes Metab J. 2024;48(1):83-96. Published online January 3, 2024; DOI: https://doi.org/10.4093/dmj.2022.0363

3,775 View
253 Download
2 Web of Science
4 Crossref

Abstract PDF Supplementary Material PubReader ePub

Background
Glucagon-like peptide-1 receptor agonist (GLP-1RA), which is a therapeutic agent for the treatment of type 2 diabetes mellitus, has a beneficial effect on the cardiovascular system.
Methods
To examine the protective effects of GLP-1RAs on proliferation and migration of vascular smooth muscle cells (VSMCs), A-10 cells exposed to angiotensin II (Ang II) were treated with either exendin-4, liraglutide, or dulaglutide. To examine the effects of GLP-1RAs on vascular calcification, cells exposed to high concentration of inorganic phosphate (Pi) were treated with exendin-4, liraglutide, or dulaglutide.
Results
Ang II increased proliferation and migration of VSMCs, gene expression levels of Ang II receptors AT1 and AT2, proliferation marker of proliferation Ki-67 (Mki-67), proliferating cell nuclear antigen (Pcna), and cyclin D1 (Ccnd1), and the protein expression levels of phospho-extracellular signal-regulated kinase (p-Erk), phospho-c-JUN N-terminal kinase (p-JNK), and phospho-phosphatidylinositol 3-kinase (p-Pi3k). Exendin-4, liraglutide, and dulaglutide significantly decreased the proliferation and migration of VSMCs, the gene expression levels of Pcna, and the protein expression levels of p-Erk and p-JNK in the Ang II-treated VSMCs. Erk inhibitor PD98059 and JNK inhibitor SP600125 decreased the protein expression levels of Pcna and Ccnd1 and proliferation of VSMCs. Inhibition of GLP-1R by siRNA reversed the reduction of the protein expression levels of p-Erk and p-JNK by exendin-4, liraglutide, and dulaglutide in the Ang II-treated VSMCs. Moreover, GLP-1 (9-36) amide also decreased the proliferation and migration of the Ang II-treated VSMCs. In addition, these GLP-1RAs decreased calcium deposition by inhibiting activating transcription factor 4 (Atf4) in Pi-treated VSMCs.
Conclusion
These data show that GLP-1RAs ameliorate aberrant proliferation and migration in VSMCs through both GLP-1Rdependent and independent pathways and inhibit Pi-induced vascular calcification.

Citations

Citations to this article as recorded by

Incretin Hormone Secretion in Women with Polycystic Ovary Syndrome: Roles of Obesity, Insulin Sensitivity and Treatment with Metformin and GLP-1s
Andrea Etrusco, Mislav Mikuš, Antonio D’Amato, Fabio Barra, Petar Planinić, Trpimir Goluža, Giovanni Buzzaccarini, Jelena Marušić, Mara Tešanović, Antonio Simone Laganà
Biomedicines.2024; 12(3): 653. CrossRef
Cardiometabolic Crossroads: Obesity, Sleep-Disordered Breathing, and Epicardial Adipose Tissue in Heart Failure with Preserved Ejection Fraction – A Mini-Review
Fulvio Cacciapuoti, Ciro Mauro, Valentina Capone, Angelo Sasso, Luca Gaetano Tarquinio, Federico Cacciapuoti
Heart and Mind.2024;[Epub] CrossRef
BRCC36 regulates β-catenin ubiquitination to alleviate vascular calcification in chronic kidney disease
Yalan Li, Xiaoyue Chen, Yiqing Xiong, Xueqiang Xu, Caidie Xie, Min Min, Dongmei Liang, Cheng Chen, Huijuan Mao
Journal of Translational Medicine.2024;[Epub] CrossRef
Perioperative Glucagon-Like Peptide-1 Agonist Use and Rates of Pseudarthrosis After Single-Level Lumbar Fusion: A Large Retrospective Cohort Study
Vedant Agrawal, Saketh Amasa, Mert Karabacak, Konstantinos Margetis
Neurosurgery.2024;[Epub] CrossRef

Review

The Role of Oxidative Stress in the Pathogenesis of Diabetic Vascular Complications: Shuji Sasaki, Toyoshi Inoguchi; Diabetes Metab J. 2012;36(4):255-261. Published online August 20, 2012; DOI: https://doi.org/10.4093/dmj.2012.36.4.255

4,625 View
70 Download
61 Crossref

Abstract PDF PubReader

Oxidative stress has been paid increasing attention to as an important causative factor for diabetic vascular complications. Among possible various sources, accumulating evidence has indicated that NAD(P)H oxidase may be the most important source for reactive oxygen species production in diabetic vascular tissues. The mechanisms underlying activation and up-regulation of NAD(P)H oxidase has been supposed to be mediated by high glucose-induced protein kinase C (PKC) activation. In this review article, activation of local renin-angiotensin II system induced by chymase activation is also shown to amplify such a PKC-dependent activation of NAD(P)H oxidase. Additionally, human evidence showing the beneficial effect of antioxidants on diabetic vascular complications. Bilirubin has been recognized as a strong endogenous antioxidant. Here markedly lower prevalence of vascular complications is shown in diabetic patients with Gilbert syndrome, a congenital hyperbilirubinemia, as well as reduced markers of oxidative stress and inflammation. Lastly, statin, angiotensin II receptor blocker, chymase inhibitor, bilirubin and biliverdin, PKC β isoform inhibitor, and glucagon-like peptide-1 analog, are shown to serve as antioxidants and have some beneficial effect on diabetic vascular complications, via inhibiting PKC-NAD(P)H oxidase activation, supporting the notion that this mechanism may be an effective therapeutic target for preventing diabetic vascular complications.

Citations

Citations to this article as recorded by

The effects of whey protein on anthropometric parameters, resting energy expenditure, oxidative stress, and appetite in overweight/obese women with type 2 diabetes mellitus: A randomized placebo controlled clinical trial
Maryam Nouri, Bahram Pourghassem Gargari, Zahra Ghasempour, Vahideh Sadra, Mohammad Asghari Jafarabadi, Arvin Babaei, Pedram Tajfar, Ali Tarighat-Esfanjani
International Journal of Diabetes in Developing Countries.2024; 44(1): 155. CrossRef
Rhodanine-benzamides as potential hits for α-amylase enzyme inhibitors and radical (DPPH and ABTS) scavengers
Samuel Attah Egu, Irfan Ali, Khalid Mohammed Khan, Sridevi Chigurupati, Urooj Qureshi, Uzma Salar, Zaheer Ul-Haq, Suliman A. Almahmoud, Shatha Ghazi Felemban, Mohsin Ali, Muhammad Taha
Molecular Diversity.2024;[Epub] CrossRef
In Vitro Models of Diabetes: Focus on Diabetic Retinopathy
Giulia Galgani, Giorgia Bray, Alma Martelli, Vincenzo Calderone, Valentina Citi
Cells.2024; 13(22): 1864. CrossRef
Research Progress of FGF-21 and CVAI in Type 2 Diabetic Kidney Disease
央宗才巴
Advances in Clinical Medicine.2023; 13(07): 12056. CrossRef
Is Subcutaneous Rifamycin Application Superior to Saline Application in Hip Hemiarthroplasty?
Malik Çelik, Nezih Ziroğlu, Alkan Bayrak
Bakirkoy Tip Dergisi / Medical Journal of Bakirkoy.2023; 19(2): 186. CrossRef
In pursuit of incorporation of markers of oxidative stress in traditional biochemical panels in clinical Chemistry: A risk assessment step in diagnosis and biotherapy
John Ibhagbemien Anetor, Chukwuemelie Zedech Uche, Gloria Oiyahumen Anetor
American Journal of Biopharmacy and Pharmaceutical Sciences.2022; 2: 1. CrossRef
Pathophysiologic Mechanisms and Potential Biomarkers in Diabetic Kidney Disease
Chan-Young Jung, Tae-Hyun Yoo
Diabetes & Metabolism Journal.2022; 46(2): 181. CrossRef
p38 MAPK Inhibitor (SB203580) and Metformin Reduces Aortic Protein Carbonyl and Inflammation in Non-obese Type 2 Diabetic Rats
Nuttikarn Nokkaew, Podsawee Mongkolpathumrat, Ruttanapong Junsiri, Supawit Jindaluang, Nichagron Tualamun, Niya Manphatthanakan, Nareumon Saleesee, Marisa Intasang, Jantira Sanit, Punyanuch Adulyaritthikul, Kantapich Kongpol, Sarawut Kumphune, Nitirut Ner
Indian Journal of Clinical Biochemistry.2021; 36(2): 228. CrossRef
Resveratrol-Elicited PKC Inhibition Counteracts NOX-Mediated Endothelial to Mesenchymal Transition in Human Retinal Endothelial Cells Exposed to High Glucose
Roberta Giordo, Gheyath K. Nasrallah, Anna Maria Posadino, Francesco Galimi, Giampiero Capobianco, Ali Hussein Eid, Gianfranco Pintus
Antioxidants.2021; 10(2): 224. CrossRef
BIOLOGICAL ACTIVE COMPONENTS OF SILENE TOMENTELLA AND THEIR PHARMACOLOGICAL PROPERTIES
Ugiloy Yusufovna Yusupova, Nurmurod Sheralievich Ramazonov, Khairulla Mamadievich Bobakulov, Feruza Rustamovna Egamova, Vladimir Nikolaevich Syrov, Durbek Abdikhoshimovich Usmanov
chemistry of plant raw material.2021; (1): 197. CrossRef
Chronically Elevated Exogenous Glucose Elicits Antipodal Effects on the Proteome Signature of Differentiating Human iPSC-Derived Pancreatic Progenitors
Luiza Ghila, Thomas Aga Legøy, Andreas Frøslev Mathisen, Shadab Abadpour, Joao A. Paulo, Hanne Scholz, Helge Ræder, Simona Chera
International Journal of Molecular Sciences.2021; 22(7): 3698. CrossRef
Effect of Type-2 Diabetes Mellitus in Retinopathy Patients on MDA, SOD Activity and its Correlation with HbA1c
Yali Hou, Mei Lin, Xuan Qiu, Mingjuan He, Yu Zhang, Feifei Guo
Brazilian Archives of Biology and Technology.2021;[Epub] CrossRef
Ameliorative effects of Hydrolea zeylanica in streptozotocin-induced oxidative stress and metabolic changes in diabetic rats
Sandeep Kumar Swain, Umesh Chandra Dash, Satish Kanhar, Atish Kumar Sahoo
Journal of Ethnopharmacology.2020; 247: 112257. CrossRef
Imbalance in the antioxidant defence system and pro-genotoxic status induced by high glucose concentrations: In vitro testing in human liver cells
Mattia Acito, Desirée Bartolini, Maria Rachele Ceccarini, Carla Russo, Samuele Vannini, Luca Dominici, Michela Codini, Milena Villarini, Francesco Galli, Tommaso Beccari, Massimo Moretti
Toxicology in Vitro.2020; 69: 105001. CrossRef
Synthesis of sulpha drug based hydroxytriazene derivatives: Anti-diabetic, antioxidant, anti-inflammatory activity and their molecular docking studies
Poonam Sharma, Varsha Dayma, Aparna Dwivedi, Prabhat K. Baroliya, I.P. Tripathi, Murugesan Vanangamudi, R.S. Chauhan, A.K. Goswami
Bioorganic Chemistry.2020; 96: 103642. CrossRef
Analysis of long non-coding RNA expression profiles in high-glucose treated vascular endothelial cells
Erqin Xu, Xiaolei Hu, Xiaoli Li, Guoxi Jin, Langen Zhuang, Qiong Wang, Xiaoyan Pei
BMC Endocrine Disorders.2020;[Epub] CrossRef
Potent α-amylase inhibitors and radical (DPPH and ABTS) scavengers based on benzofuran-2-yl(phenyl)methanone derivatives: Syntheses, in vitro, kinetics, and in silico studies
Irfan Ali, Rafaila Rafique, Khalid Mohammed Khan, Sridevi Chigurupati, Xingyue Ji, Abdul Wadood, Ashfaq Ur Rehman, Uzma Salar, Muhammad Shahid Iqbal, Muhammad Taha, Shahnaz Perveen, Basharat Ali
Bioorganic Chemistry.2020; 104: 104238. CrossRef
A review of fibroblast growth factor 21 in diabetic cardiomyopathy
Xiang Zhang, Luo Yang, Xiongfeng Xu, Fengjuan Tang, Peng Yi, Bo Qiu, Yarong Hao
Heart Failure Reviews.2019; 24(6): 1005. CrossRef
Recent advances in the pathogenesis of microvascular complications in diabetes
Sungmi Park, Hyeon-Ji Kang, Jae-Han Jeon, Min-Ji Kim, In-Kyu Lee
Archives of Pharmacal Research.2019; 42(3): 252. CrossRef
Metabolic syndrome status over 2 years predicts incident chronic kidney disease in mid-life adults: a 10-year prospective cohort study
So Jin Lee, Hun Ju Lee, Hyun jeong Oh, Taehwa Go, Dae Ryong Kang, Jang Young Kim, Ji Hye Huh
Scientific Reports.2018;[Epub] CrossRef
Role of peroxisome proliferator–activated receptor-gamma activation on visfatin, advanced glycation end products, and renal oxidative stress in obesity-induced type 2 diabetes mellitus
A Tabassum, T Mahboob
Human & Experimental Toxicology.2018; 37(11): 1187. CrossRef
Coffee consumption and reduced risk of developing type 2 diabetes: a systematic review with meta-analysis
Mattias Carlström, Susanna C Larsson
Nutrition Reviews.2018; 76(6): 395. CrossRef
Liraglutide ameliorates early renal injury by the activation of renal FoxO1 in a type 2 diabetic kidney disease rat model
Pin Chen, Xiaozhi Shi, Xiangjin Xu, Yiyang Lin, Zhulin Shao, Rongdan Wu, Lihong Huang
Diabetes Research and Clinical Practice.2018; 137: 173. CrossRef
Antioxidant effects of vitamins in type 2 diabetes: a meta-analysis of randomized controlled trials
Maria E. Balbi, Fernanda S. Tonin, Antonio M. Mendes, Helena H. Borba, Astrid Wiens, Fernando Fernandez-Llimos, Roberto Pontarolo
Diabetology & Metabolic Syndrome.2018;[Epub] CrossRef
Gilbert syndrome in patients with type 1 diabetes-Prevalence, glycemic control, and microalbuminuria
Sigal Singer, Nurit Pilpel, Orit Pinhas-Hamiel
Pediatric Diabetes.2017; 18(8): 803. CrossRef
Low serum bilirubin level predicts the development of chronic kidney disease in patients with type 2 diabetes mellitus
Kang Hee Ahn, Sang Soo Kim, Won Jin Kim, Jong Ho Kim, Yun Jeong Nam, Su Bin Park, Yun Kyung Jeon, Bo Hyun Kim, In Joo Kim, Yong Ki Kim
The Korean Journal of Internal Medicine.2017; 32(5): 875. CrossRef
Tiron ameliorates high glucose‐induced cardiac myocyte apoptosis by PKCδ‐dependent inhibition of osteopontin
Ping Jiang, Deling Zhang, Hong Qiu, Xianqi Yi, Yemin Zhang, Yingkang Cao, Bo Zhao, Zhongyuan Xia, Changhua Wang
Clinical and Experimental Pharmacology and Physiology.2017; 44(7): 760. CrossRef
Protective effects of 6-Gingerol on vascular endothelial cell injury induced by high glucose via activation of PI3K-AKT-eNOS pathway in human umbilical vein endothelial cells
Dan Liu, Mengqing Wu, Yi Lu, Tao Xian, Yupeng Wang, Bowei Huang, Guohua Zeng, Qiren Huang
Biomedicine & Pharmacotherapy.2017; 93: 788. CrossRef
Adenosine signaling in diabetes mellitus and associated cardiovascular and renal complications
Maria Peleli, Mattias Carlstrom
Molecular Aspects of Medicine.2017; 55: 62. CrossRef
Diabetes and increased lipid peroxidation are associated with systemic inflammation even in well-controlled patients
Alliny de Souza Bastos, Dana T. Graves, Ana Paula de Melo Loureiro, Carlos Rossa Júnior, Sâmia Cruz Tfaile Corbi, Fausto Frizzera, Raquel Mantuaneli Scarel-Caminaga, Niels Olsen Câmara, Oelisoa M. Andriankaja, Meire I. Hiyane, Silvana Regina Perez Orrico
Journal of Diabetes and its Complications.2016; 30(8): 1593. CrossRef
Agkistrodon acutus-purified protein C activator protects human umbilical vein endothelial cells against H2O2-induced apoptosis
Shu Li, Yun Hong, Xin Jin, Xianwei Li, Entao Sun, Genbao Zhang, Linming Lu, Liuwang Nie
Pharmaceutical Biology.2016; 54(12): 3285. CrossRef
The impact of mast cells on cardiovascular diseases
Eva Kritikou, Johan Kuiper, Petri T. Kovanen, Ilze Bot
European Journal of Pharmacology.2016; 778: 103. CrossRef
Comparison of acarbose and metformin therapy in newly diagnosed type 2 diabetic patients with overweight and/or obesity
Weiping Sun, Chunping Zeng, Lizhen Liao, Juan Chen, Ying Wang
Current Medical Research and Opinion.2016; 32(8): 1389. CrossRef
Mechanisms for the cardiovascular effects of glucagon-like peptide-1
H. Poudyal
Acta Physiologica.2016; 216(3): 277. CrossRef
Integrated scientific data bases review on asulacrine and associated toxicity
Attia Afzal, Muhammad Sarfraz, Zimei Wu, Guangji Wang, Jianguo Sun
Critical Reviews in Oncology/Hematology.2016; 104: 78. CrossRef
Impact of Diabetes and Defective Thyroid Ontogenesis on Audition
Luciene da Cruz Fernandes
Journal of Diabetes, Metabolic Disorders & Control.2016;[Epub] CrossRef
Serum Total Bilirubin Levels Provide Additive Risk Information over the Framingham Risk Score for Identifying Asymptomatic Diabetic Patients at Higher Risk for Coronary Artery Stenosis
Jaechan Leem, Eun Hee Koh, Jung Eun Jang, Chang-Yun Woo, Jin Sun Oh, Min Jung Lee, Joon-Won Kang, Tae-Hwan Lim, Chang Hee Jung, Woo Je Lee, Joong-Yeol Park, Ki-Up Lee
Diabetes & Metabolism Journal.2015; 39(5): 414. CrossRef
Clinical implications of carotid artery intima media thickness assessment on cardiovascular risk stratification in hyperlipidemic Korean adults with diabetes: the ALTO study
Eun-Gyoung Hong, Jung Hun Ohn, Seong Jin Lee, Hyuk Sang Kwon, Sin Gon Kim, Dong Jun Kim, Dong Sun Kim
BMC Cardiovascular Disorders.2015;[Epub] CrossRef
Undiagnosed diabetes is prevalent in younger adults and associated with a higher risk cardiometabolic profile compared to diagnosed diabetes
Yong-ho Lee, Ehrin J. Armstrong, Gyuri Kim, Jaewon Oh, Seok-Min Kang, Byung-Wan Lee, Chul Woo Ahn, Bong Soo Cha, Hyun Chul Lee, Christos S. Mantzoros, Eun Seok Kang
American Heart Journal.2015; 170(4): 760. CrossRef
Common Polymorphisms in Antioxidant Genes are Associated with Diabetic Nephropathy in Type 2 Diabetes Patients
Jasna Klen, Katja Goričar, Andrej Janež, Vita Dolžan
Personalized Medicine.2015; 12(3): 187. CrossRef
Increased endogenous serotonin level in diabetic conditions may lead to cardiac valvulopathy via reactive oxygen species regulation
Tahir Ali, Hina Waheed, Farhat Shaheen, Madiha Mahmud, Qamar Javed, Iram Murtaza
Biologia.2015; 70(2): 273. CrossRef
Antioxidant Effects of Statins in the Management of Cardiometabolic Disorders
Soo Lim, Philip Barter
Journal of Atherosclerosis and Thrombosis.2014; 21(10): 997. CrossRef
Evidence-Based Practice Use of Incretin-Based Therapy in the Natural History of Diabetes
Stanley Schwartz
Postgraduate Medicine.2014; 126(3): 66. CrossRef
Low serum bilirubin concentration is a novel risk factor for the development of albuminuria in patients with type 2 diabetes
Hiroshi Okada, Michiaki Fukui, Muhei Tanaka, Shinobu Matsumoto, Kanae Kobayashi, Hiroya Iwase, Kiichiro Tomiyasu, Koji Nakano, Goji Hasegawa, Naoto Nakamura
Metabolism.2014; 63(3): 409. CrossRef
Serum total bilirubin levels and prevalence of diabetic retinopathy in a Chinese population (中国人群血清总胆红素水平与糖尿病视网膜病变相关性的研究)
Syeda Sadia Najam, Jichao Sun, Jie Zhang, Min Xu, Jieli Lu, Kan Sun, Mian Li, Tiange Wang, Yufang Bi, Guang Ning
Journal of Diabetes.2014; 6(3): 221. CrossRef
Roles of oxidative stress, adiponectin, and nuclear hormone receptors in obesity-associated insulin resistance and cardiovascular risk
Morihiro Matsuda, Iichiro Shimomura
Hormone Molecular Biology and Clinical Investigation.2014;[Epub] CrossRef
Unilateral injection of Aβ25–35 in the hippocampus reduces the number of dendritic spines in hyperglycemic rats
Zayda Lazcano, Oscar Solis, María Elena Bringas, Daniel Limón, Alfonso Diaz, Blanca Espinosa, Isabel García‐Peláez, Gonzalo Flores, Jorge Guevara
Synapse.2014; 68(12): 585. CrossRef
Role of angiotensin-converting enzyme 2 (ACE2) in diabetic cardiovascular complications
Vaibhav B. Patel, Nirmal Parajuli, Gavin Y. Oudit
Clinical Science.2014; 126(7): 471. CrossRef
Pathogenesis of Chronic Hyperglycemia: From Reductive Stress to Oxidative Stress
Liang-Jun Yan
Journal of Diabetes Research.2014; 2014: 1. CrossRef
Daily intake of vitamin D‐ or calcium‐vitamin D‐fortified Persian yogurt drink (doogh) attenuates diabetes‐induced oxidative stress: evidence for antioxidative properties of vitamin D
B. Nikooyeh, T. R. Neyestani, N. Tayebinejad, H. Alavi‐Majd, N. Shariatzadeh, A. Kalayi, M. Zahedirad, S. Heravifard, S. Salekzamani
Journal of Human Nutrition and Dietetics.2014; 27(s2): 276. CrossRef
Roles of adiponectin and oxidative stress in obesity-associated metabolic and cardiovascular diseases
Morihiro Matsuda, Iichiro Shimomura
Reviews in Endocrine and Metabolic Disorders.2014; 15(1): 1. CrossRef
PKCδ-dependent Activation of the Ubiquitin Proteasome System is Responsible for High Glucose-induced Human Breast Cancer MCF-7 Cell Proliferation, Migration and Invasion
Shan Zhu, Feng Yao, Wen-Huan Li, Jin-Nan Wan, Yi-Min Zhang, Zhao Tang, Shahzad Khan, Chang-Hua Wang, Sheng-Rong Sun
Asian Pacific Journal of Cancer Prevention.2013; 14(10): 5687. CrossRef
Increased oxidative stress in obesity: Implications for metabolic syndrome, diabetes, hypertension, dyslipidemia, atherosclerosis, and cancer
Morihiro Matsuda, Iichiro Shimomura
Obesity Research & Clinical Practice.2013; 7(5): e330. CrossRef
Hydrogen Sulfide Suppresses High Glucose–Induced Expression of Intercellular Adhesion Molecule-1 in Endothelial Cells
Qingbo Guan, Xiaolei Wang, Ling Gao, Jicui Chen, Yuantao Liu, Chunxiao Yu, Nan Zhang, Xu Zhang, Jiajun Zhao
Journal of Cardiovascular Pharmacology.2013; 62(3): 278. CrossRef
Clinical Marker of Platelet Hyperreactivity in Diabetes Mellitus
Jin Hwa Kim, Hak Yeon Bae, Sang Yong Kim
Diabetes & Metabolism Journal.2013; 37(6): 423. CrossRef
Glucose initially inhibits and later stimulates blood ROS generation
Thomas Stief
Journal of Diabetes Mellitus.2013; 03(01): 15. CrossRef
Oxidative Stress in Cardiovascular Diseases and Obesity: Role of p66Shc and Protein Kinase C
Elena De Marchi, Federica Baldassari, Angela Bononi, Mariusz R. Wieckowski, Paolo Pinton
Oxidative Medicine and Cellular Longevity.2013; 2013: 1. CrossRef
Toxicological and pharmacological concerns on oxidative stress and related diseases
Soodabeh Saeidnia, Mohammad Abdollahi
Toxicology and Applied Pharmacology.2013; 273(3): 442. CrossRef
Comparison of antioxidant, α-glucosidase inhibition and anti-inflammatory activities of the leaf and root extracts ofSmilax chinaL.
Kyoung Kon Kim, Yun Hwan Kang, Dae Jung Kim, Tae Woo Kim, Myeon Choe
Journal of Nutrition and Health.2013; 46(4): 315. CrossRef
Correlation between blood glucose fluctuations and activation of oxidative stress in type 1 diabetic children during the acute metabolic disturbance period
Di WU, Chun-xiu GONG, Xi MENG, Qiu-lan YANG
Chinese Medical Journal.2013; 126(21): 4019. CrossRef
Oxidative stress and its complications in human health
Guniz M. Koksal
Advances in Bioscience and Biotechnology.2012; 03(08): 1113. CrossRef

Original Articles

Angiotensin II Inhibits Insulin Binding to Endothelial Cells: Su-Jin Oh, Won-Chul Ha, Jee-In Lee, Tae-Seo Sohn, Ji-Hyun Kim, Jung-Min Lee, Sang-Ah Chang, Oak-Kee Hong, Hyun-Shik Son; Diabetes Metab J. 2011;35(3):243-247. Published online June 30, 2011; DOI: https://doi.org/10.4093/dmj.2011.35.3.243

4,375 View
27 Download
6 Crossref

Abstract PDF PubReader

Background

Insulin-mediated glucose uptake in insulin target tissues is correlated with interstitial insulin concentration, rather than plasma insulin concentration. Therefore, insulin delivery to the interstitium of target tissues is very important, and the endothelium may also play an important role in the development of insulin resistance.

Methods

After treating bovine aortic endothelial cells with angiotensin II (ATII), we observed the changes in insulin binding capacity and the amounts of insulin receptor (IR) on the cell membranes and in the cytosol.

Results

After treatment of 10^-7M ATII, insulin binding was decreased progressively, up to 60% at 60 minutes (P<0.05). ATII receptor blocker (eprosartan) dose dependently improved the insulin binding capacity which was reduced by ATII (P<0.05). At 200 µM, eprosartan fully restored insulin binding capacity, althogh it resulted in only a 20% to 30% restoration at the therapeutic concentration. ATII did not affect the total amount of IR, but it did reduce the amount of IR on the plasma membrane and increased that in the cytosol.

Conclusion

ATII decreased the insulin binding capacity of the tested cells. ATII did not affect the total amount of IR but did decrease the amount of IR on the plasma membrane. Our data indicate that ATII decreases insulin binding by translocating IR from the plasma membrane to the cytosol. The binding of insulin to IR is important for insulin-induced vasodilation and transendothelial insulin transport. Therefore, ATII may cause insulin resistance through this endothelium-based mechanism.

Citations

Citations to this article as recorded by

Acute, local infusion of angiotensin II impairs microvascular and metabolic insulin sensitivity in skeletal muscle
Dino Premilovac, Emily Attrill, Stephen Rattigan, Stephen M Richards, Jeonga Kim, Michelle A Keske
Cardiovascular Research.2019; 115(3): 590. CrossRef
Angiotensin II type 2 receptor inhibits expression and function of insulin receptor in rat renal proximal tubule cells
Yang Yang, Caiyu Chen, Chunjiang Fu, Zaicheng Xu, Cong Lan, Yongchun Zeng, Zhi Chen, Pedro A. Jose, Ye Zhang, Chunyu Zeng
Journal of the American Society of Hypertension.2018; 12(2): 135. CrossRef
Endothelial function, its relation to arterial hypertension and the possibility of its modulation
Vladislav Biel, Jan Novák, Luděk Pluháček, Jiří Špác
Vnitřní lékařství.2018; 64(7-8): 762. CrossRef
Evidence to Consider Angiotensin II Receptor Blockers for the Treatment of Early Alzheimer’s Disease
Juan M. Saavedra
Cellular and Molecular Neurobiology.2016; 36(2): 259. CrossRef
Ameliorative effect of eprosartan on high-fat diet/streptozotocin-induced early diabetic nephropathy in rats
Mohamed A. Morsy, Gehan H. Heeba, Magda E. Mahmoud
European Journal of Pharmacology.2015; 750: 90. CrossRef
Metabolic actions of angiotensin II and insulin: A microvascular endothelial balancing act
Ranganath Muniyappa, Sahzene Yavuz
Molecular and Cellular Endocrinology.2013; 378(1-2): 59. CrossRef

Effect of Peroxisome Proliferator Activated Receptor-gamma Agonist, Angiotensin II Receptor Blocker and alpha-lipoic Acid on Renal VEGF Expression in Diabetic Nephropathy.: Jang Hyun Koh, Yeon Lee, Mi Jin Kim, Young Goo Shin, Eun Young Lee, Choon Hee Chung; Korean Diabetes J. 2004;28(5):367-376. Published online October 1, 2004

1,245 View
34 Download

Abstract PDF: BACKGROUND
Diabetic nephropathy is one of the most serious complications in diabetes mellitus, and it is the leading cause of end stage renal disease. It has been reported that angiotensin converting enzyme inhibitor (ACEi) reduces the vascular endothelial growth factor (VEGF) expression, and so it plays an important role in reducing the renal damage. Peroxisome proliferator activated receptor-gamma (PPAR-gamma) agonist is known to reduce insulin resistance in type 2 diabetic patients. In the previous study, PPAR-gamma agonist was shown to lower VEGF expression in the retina, but it increased the plasma VEGF level. Alpha-lipoic acid (alpha-LA), which is an antioxidant, lowers the increased level of VEGF in retina as well. The precise role of PPAR-gamma agonist and alpha-LA on renal VEGF expression in diabetic nephropathy is still uncertain. We studied the effect of PPAR-gamma agonist, angiotensin II receptor blocker (ATIIRB) and alpha-LA on the renal VEGF expression in diabetic rats. METHODS: We used 60 Sprague-Dawley male rats, those were 8 weeks old and weighted about 300 g each as the study subjects. Among them, 48 rats were chosen and injected with streptozotocin (70 mg/kg) into peritoneal cavity to induce diabetes mellitus. The rast were than divided into 5 groups. Group I was a normal control group (n=12), group II was diabetic control group (n=12), group III was diabetic group that was given with PPAR-gamma agonist (n=12), group IV was the diabetic group that was given ATIIRB (n=12), and group V was the diabetic rats that were given alpha-LA (n=12). We measured their body weight, blood glucose levels, 24 hour urine protein and albumin levels at the baseline, the 8th and the 16th weeks of the experiment. On the 16th weeks of our experiment we extracted the kidneys to measure the glomerular volume, the optical density of the VEGF staining and VEGF mRNA expression. RESULTS: At the beginning of the study, the 5 groups all showed similar 24 hour urine albumin levels. At the 8th week, group II showed an increased urine albumin level of 143.4 +/- 117.2 mg/day; this was greater than that of group IV (60.7+/-30.6 mg/day) (p<0.05). The glomerular volume and optical densities of VEGF expression were significantly reduced in group III, IV and V compared to group II. For group IV and V, the renal VEGF mRNA expression was significantly lower than that of group II, but group III showed no significant difference. from group II. CONCLUSION: Angiotensin II receptor blocker delayed the progression of diabetic nephropathy. PPAR-gamma agonist and alpha-lipoic acid did not have any protective effect against the progression of diabetic nephropathy in spite of the decreased VEGF expression noted in this study.

Effect of Angiotensin II Receptor Blockade on VEGF Expression in Diabetic Nephropathy.: Myoungsook Shim, Mijin Kim, Munkyu Kim, Hyunjin Chang, Younggoo Shin, Junam Kim, Jaeman Song, Hosuk Kang, Eunyoung Lee, Kihak Song, Choonhee Chung; Korean Diabetes J. 2003;27(2):106-114. Published online April 1, 2003

1,130 View
20 Download

Abstract PDF: BACKGROUND
Diabetic nephropathy is one of the most serious complications of diabetes, and is the leading cause of chronic renal failure. Vascular endothelial growth factor (VEGF) plays an important role in the pathophysiology of diabetic retinopathy, which can be blocked by ACE inhibitors, but its precise role in diabetic nephropathy is uncertain. METHODS: 32 eight week-old Sprague-Dawley male rats were prepared, of which 16 were chosen for injection with streptozotocin (60 mg/kg) into the peritoneal cavity, with the goal of inducing diabetes. One week later, the peripheral blood sugar, taken from the tail vein was checked. A glucose level exceeding 200 mg/dL was taken as evidence of diabetes. The rats were divided into 4 groups of 8. Group I served as a control. Group II was treated with angiotensin II receptor blockade (L-158,809, 5 mg/kg/day, in drinking water). Group III consisted of diabetic rats and group IV diabetic rats treated with the same angiotensin II receptor blockade (L-158,809). At the beginning of the experiment and on 8th and 12th weeks, 24-hour urine protein and body weight checks were performed. At the end of the study, I extracted kidney and the glomerular volumes and optical densities of the VEGF expression in the glomeruli compared. RESULTS: The basal characteristics were initially the same. However, on weeks 8 and 12 the amount of 24-hour urine protein had increased in groups III and IV (p<0.05). By week 12, it was noticeably greater in group III than in group IV (p<0.05). The glomerular volume was also greater in groups III and IV (p<0.05). Optical density of the VEGF in the glomeruli had increased more in group III than in groups I, II and IV (p<0.05). CONCLUSION: VEGF plays a precise role in diabetic nephropathy, and angiotensin II receptor blockade can reduce diabetic nephropathy by suppressing the expression of VEGF.

First
Prev
Page of 1
Next
Last

Search