- Basic Research
- Extracellular Vimentin Alters Energy Metabolism And Induces Adipocyte Hypertrophy
-
Ji-Hae Park, Soyeon Kwon, Young Mi Park
-
Diabetes Metab J. 2024;48(2):215-230. Published online September 26, 2023
-
DOI: https://doi.org/10.4093/dmj.2022.0332
-
-
4,514
View
-
321
Download
-
3
Web of Science
-
4
Crossref
-
 Abstract
 PDF Supplementary Material PubReader  ePub
- Background
Previous studies have reported that oxidative stress contributes to obesity characterized by adipocyte hypertrophy. However, mechanism has not been studied extensively. In the current study, we evaluated role of extracellular vimentin secreted by oxidized low-density lipoprotein (oxLDL) in energy metabolism in adipocytes.
Methods
We treated 3T3-L1-derived adipocytes with oxLDL and measured vimentin which was secreted in the media. We evaluated changes in uptake of glucose and free fatty acid, expression of molecules functioning in energy metabolism, synthesis of adenosine triphosphate (ATP) and lactate, markers for endoplasmic reticulum (ER) stress and autophagy in adipocytes treated with recombinant vimentin.
Results
Adipocytes secreted vimentin in response to oxLDL. Microscopic evaluation revealed that vimentin treatment induced increase in adipocyte size and increase in sizes of intracellular lipid droplets with increased intracellular triglyceride. Adipocytes treated with vimentin showed increased uptake of glucose and free fatty acid with increased expression of plasma membrane glucose transporter type 1 (GLUT1), GLUT4, and CD36. Vimentin treatment increased transcription of GLUT1 and hypoxia-inducible factor 1α (Hif-1α) but decreased GLUT4 transcription. Adipose triglyceride lipase (ATGL), peroxisome proliferator-activated receptor γ (PPARγ), sterol regulatory element-binding protein 1 (SREBP1), diacylglycerol O-acyltransferase 1 (DGAT1) and 2 were decreased by vimentin treatment. Markers for ER stress were increased and autophagy was impaired in vimentin-treated adipocytes. No change was observed in synthesis of ATP and lactate in the adipocytes treated with vimentin.
Conclusion
We concluded that extracellular vimentin regulates expression of molecules in energy metabolism and promotes adipocyte hypertrophy. Our results show that vimentin functions in the interplay between oxidative stress and metabolism, suggesting a mechanism by which adipocyte hypertrophy is induced in oxidative stress.
-
Citations
Citations to this article as recorded by  - Novel secreted regulators of glucose and lipid metabolism in the development of metabolic diseases
Lianna W. Wat, Katrin J. Svensson
Diabetologia.2024; 67(12): 2626. CrossRef - Mechanobiology in Metabolic Dysfunction-Associated Steatotic Liver Disease and Obesity
Emily L. Rudolph, LiKang Chin
Current Issues in Molecular Biology.2024; 46(7): 7134. CrossRef - Context-specific fatty acid uptake is a finely-tuned multi-level effort
Juan Wang, Huiling Guo, Lang-Fan Zheng, Peng Li, Tong-Jin Zhao
Trends in Endocrinology & Metabolism.2024;[Epub] CrossRef - The Functions of SARS-CoV-2 Receptors in Diabetes-Related Severe COVID-19
Adam Drzymała
International Journal of Molecular Sciences.2024; 25(17): 9635. CrossRef
- Basic Research
- Vimentin Deficiency Prevents High-Fat Diet-Induced Obesity and Insulin Resistance in Mice
-
SeoYeon Kim, Inyeong Kim, Wonkyoung Cho, Goo Taeg Oh, Young Mi Park
-
Diabetes Metab J. 2021;45(1):97-108. Published online June 15, 2020
-
DOI: https://doi.org/10.4093/dmj.2019.0198
-
-
8,846
View
-
252
Download
-
19
Web of Science
-
20
Crossref
-
 Graphical Abstract
Abstract
PDFSupplementary MaterialPubReader ePub
 -
Background
Obesity and type 2 diabetes mellitus are world-wide health problems, and lack of understanding of their linking mechanism is one reason for limited treatment options. We determined if genetic deletion of vimentin, a type 3 intermediate filament, affects obesity and type 2 diabetes mellitus.
Methods
We fed vimentin-null (Vim−/−) mice and wild-type mice a high-fat diet (HFD) for 10 weeks and measured weight change, adiposity, blood lipids, and glucose. We performed intraperitoneal glucose tolerance tests and measured CD36, a major fatty acid translocase, and glucose transporter type 4 (GLUT4) in adipocytes from both groups of mice.
Results
Vim−/− mice fed an HFD showed less weight gain, less adiposity, improved glucose tolerance, and lower serum level of fasting glucose. However, serum triglyceride and non-esterified fatty acid levels were higher in Vim−/− mice than in wild-type mice. Vimentin-null adipocytes showed 41.1% less CD36 on plasma membranes, 27% less uptake of fatty acids, and 50.3% less GLUT4, suggesting defects in intracellular trafficking of these molecules.
Conclusion
We concluded that vimentin deficiency prevents obesity and insulin resistance in mice fed an HFD and suggest vimentin as a central mediator linking obesity and type 2 diabetes mellitus.
-
Citations
Citations to this article as recorded by - Extracellular Vimentin Alters Energy Metabolism And Induces Adipocyte Hypertrophy
Ji-Hae Park, Soyeon Kwon, Young Mi Park
Diabetes & Metabolism Journal.2024; 48(2): 215. CrossRef - Neutrophils display distinct post-translational modifications in response to varied pathological stimuli
Pooja Yedehalli Thimmappa, Aswathy S Nair, Sian D'silva, Anjana Aravind, Sandeep Mallya, Sreelakshmi Pathappillil Soman, Kanive Parashiva Guruprasad, Shamee Shastry, Rajesh Raju, Thottethodi Subrahmanya Keshava Prasad, Manjunath B Joshi
International Immunopharmacology.2024; 132: 111950. CrossRef - A Systematic Review of Proteomics in Obesity: Unpacking the Molecular Puzzle
Alba Rodriguez-Muñoz, Hanieh Motahari-Rad, Laura Martin-Chaves, Javier Benitez-Porres, Jorge Rodriguez-Capitan, Andrés Gonzalez-Jimenez, Maria Insenser, Francisco J. Tinahones, Mora Murri
Current Obesity Reports.2024; 13(3): 403. CrossRef - Mechanobiology in Metabolic Dysfunction-Associated Steatotic Liver Disease and Obesity
Emily L. Rudolph, LiKang Chin
Current Issues in Molecular Biology.2024; 46(7): 7134. CrossRef - Context-specific fatty acid uptake is a finely-tuned multi-level effort
Juan Wang, Huiling Guo, Lang-Fan Zheng, Peng Li, Tong-Jin Zhao
Trends in Endocrinology & Metabolism.2024;[Epub] CrossRef - Extracellular Vesicles as Carriers of Adipokines and Their Role in Obesity
Tamara Camino, Nerea Lago-Baameiro, María Pardo
Biomedicines.2023; 11(2): 422. CrossRef - Bioinformatics and Next-Generation Data Analysis for Identification of Genes and Molecular Pathways Involved in Subjects with Diabetes and Obesity
Prashanth Ganekal, Basavaraj Vastrad, Satish Kavatagimath, Chanabasayya Vastrad, Shivakumar Kotrashetti
Medicina.2023; 59(2): 309. CrossRef - Modified Signaling of Membrane Formyl Peptide Receptors in NADPH-Oxidase Regulation in Obesity-Resistant Mice
Irina Tikhonova, Alsu Dyukina, Elvira Shaykhutdinova, Valentina Safronova
Membranes.2023; 13(3): 306. CrossRef - Plasma Cytokeratin-18 Fragment Level Reflects the Metabolic Phenotype in Obesity
Joanna Goralska, Urszula Razny, Anna Gruca, Anna Zdzienicka, Agnieszka Micek, Aldona Dembinska-Kiec, Bogdan Solnica, Malgorzata Malczewska-Malec
Biomolecules.2023; 13(4): 675. CrossRef - Blueberry and Blackberry Anthocyanins Ameliorate Metabolic Syndrome by Modulating Gut Microbiota and Short-Chain Fatty Acids Metabolism in High-Fat Diet-Fed C57BL/6J Mice
Lanlan Du, Han Lü, Yan Chen, Xiaohua Yu, Tunyu Jian, Huifang Zhao, Wenlong Wu, Xiaoqin Ding, Jian Chen, Weilin Li
Journal of Agricultural and Food Chemistry.2023; 71(40): 14649. CrossRef - An analogue of the Prolactin Releasing Peptide reduces obesity and promotes adult neurogenesis
Sara KM Jörgensen, Alena Karnošová, Simone Mazzaferro, Oliver Rowley, Hsiao-Jou Cortina Chen, Sarah J Robbins, Sarah Christofides, Florian T Merkle, Lenka Maletínská, David Petrik
EMBO Reports.2023; 25(1): 351. CrossRef - Cytoskeleton alterations in non-alcoholic fatty liver disease
João Pessoa, José Teixeira
Metabolism.2022; 128: 155115. CrossRef - Recent Advances in the Treatment of Insulin Resistance Targeting Molecular and Metabolic Pathways: Fighting a Losing Battle?
Marta Wolosowicz, Slawomir Prokopiuk, Tomasz W. Kaminski
Medicina.2022; 58(4): 472. CrossRef - Roles of vimentin in health and disease
Karen M. Ridge, John E. Eriksson, Milos Pekny, Robert D. Goldman
Genes & Development.2022; 36(7-8): 391. CrossRef - Plasma Membrane Localization of CD36 Requires Vimentin Phosphorylation; A Mechanism by Which Macrophage Vimentin Promotes Atherosclerosis
Seo Yeon Kim, Se-Jin Jeong, Ji-Hae Park, Wonkyoung Cho, Young-Ho Ahn, Youn-Hee Choi, Goo Taeg Oh, Roy L. Silverstein, Young Mi Park
Frontiers in Cardiovascular Medicine.2022;[Epub] CrossRef - Camel Proteins and Enzymes: A Growing Resource for Functional Evolution and Environmental Adaptation
Mahmoud Kandeel, Abdulla Al-Taher, Katharigatta N. Venugopala, Mohamed Marzok, Mohamed Morsy, Sreeharsha Nagaraja
Frontiers in Veterinary Science.2022;[Epub] CrossRef - Brown Adipose Tissue Sheds Extracellular Vesicles That Carry Potential Biomarkers of Metabolic and Thermogenesis Activity Which Are Affected by High Fat Diet Intervention
Tamara Camino, Nerea Lago-Baameiro, Aurelio Sueiro, Susana Belén Bravo, Iván Couto, Francisco Fernando Santos, Javier Baltar, Felipe F. Casanueva, María Pardo
International Journal of Molecular Sciences.2022; 23(18): 10826. CrossRef - Dietary tea seed saponin combined with aerobic exercise attenuated lipid metabolism and oxidative stress in mice fed a high‐fat diet (HFD)
Wenjing Cao, Keying Wang, Chanhua Liang, Yanming Su, Shuang Liu, Jiali Li, Huishan Qing, Zhen Zeng, Ling Dai, Jia‐Le Song
Journal of Food Biochemistry.2022;[Epub] CrossRef - Influence of Protein Carbonylation on Human Adipose Tissue Dysfunction in Obesity and Insulin Resistance
M. Carmen Navarro-Ruiz, M. Carmen Soler-Vázquez, Alberto Díaz-Ruiz, Juan R. Peinado, Andrea Nieto Calonge, Julia Sánchez-Ceinos, Carmen Tercero-Alcázar, Jaime López-Alcalá, Oriol A. Rangel-Zuñiga, Antonio Membrives, José López-Miranda, María M. Malagón, R
Biomedicines.2022; 10(12): 3032. CrossRef - The Role of Adipose Tissue Lipolysis in Diet-Induced Obesity: Focus on Vimentin
Eun Roh, Hye Jin Yoo
Diabetes & Metabolism Journal.2021; 45(1): 43. CrossRef
- A Survey of Diabetic Educators and Patients for the Revision of Korean Food Exchange Lists
-
Jae Won Cho, Mee Ra Kweon, Young Mi Park, Mi Hye Woo, Hye Sook Yoo, Jeong Hyun Lim, Bo Kyung Koo, Chong Hwa Kim, Hae Jin Kim, Tae Sun Park, Choong Ho Shin, Kyu Chang Won, Soo Lim, Hak Chul Jang
-
Diabetes Metab J. 2011;35(2):173-181. Published online April 30, 2011
-
DOI: https://doi.org/10.4093/dmj.2011.35.2.173
-
-
5,665
View
-
42
Download
-
6
Crossref
-
Abstract
PDFPubReader
- Background
Food exchange lists are one of the main methods of nutritional education. However, Korean food exchange lists have not been revised since 1994. Therefore, we surveyed the opinions of diabetes educators and patients with diabetes regarding the need for revision of the current food exchange lists. MethodsFor two weeks beginning on 10 March 2008, a 12-item questionnaire regarding the opinion and need for revision of the current food exchange lists was e-mailed to diabetes educators nationwide. Another 15-question survey was administered to patients with diabetes in 13 hospitals located in the Seoul and Gyeonggi regions of Korea. ResultsWe obtained survey responses from 101 diabetes educators and 209 patients; 65 (64.3%) of the educators answered that the current food exchange lists should be revised. The items that needed revision were the glycemic index, addition of new foods and reaffirmation of exchange standard amounts. The patients demanded specific education about choosing appropriate foods, a balanced meal plan, proper snacks, and dining intake. ConclusionOur survey results demonstrate the need to revise the Korean food exchange lists. This process should focus on glycemic index, the addition of new foods and reconfirmation of one exchange reference unit.
-
Citations
Citations to this article as recorded by
|
KDA
