- Basic Research
- A Novel Pancreatic Imaging Window for Stabilized Longitudinal In Vivo Observation of Pancreatic Islets in Murine Model
-
Inwon Park, Sujung Hong, Yoonha Hwang, Pilhan Kim
-
Diabetes Metab J. 2020;44(1):193-198. Published online May 29, 2019
-
DOI: https://doi.org/10.4093/dmj.2018.0268
-
-
5,691
View
-
154
Download
-
13
Web of Science
-
11
Crossref
-
Abstract
PDFSupplementary MaterialPubReader
Longitudinal imaging of murine pancreas is technically challenging due to the mechanical softness of the tissue influenced by peristalsis. Here, we report a novel pancreatic imaging window for long-term stabilized cellular-level observation of the islets in the pancreas in vivo. By spatially separating the pancreas from the bowel movement and physiologic respiration with a metal plate integrated in the imaging window, we successfully tracked the pancreatic islets up to three weeks and visualized the dumbbell-shape transformation from the single islet. This window can be a useful tool for long-term cellular-level visualization of the microstructure in the pancreas.
-
Citations
Citations to this article as recorded by
-
Apollo-NADP
+
reveals in vivo adaptation of NADPH/NADP
+
metabolism in electrically activated pancreatic β cells
Cindy V. Bui, Curtis W. Boswell, Brian Ciruna, Jonathan V. Rocheleau Science Advances.2023;[Epub] CrossRef - Intravital imaging of the functions of immune cells in the tumor microenvironment during immunotherapy
Xuwen Peng, Yuke Wang, Jie Zhang, Zhihong Zhang, Shuhong Qi Frontiers in Immunology.2023;[Epub] CrossRef - Minimizing Motion Artifacts in Intravital Microscopy Using the Sedative Effect of Dexmedetomidine
Youngkyu Kim, Minju Cho, Bjorn Paulson, Sung-Hoon Kim, Jun Ki Kim Microscopy and Microanalysis.2022; 28(5): 1679. CrossRef - SWIP—a stabilized window for intravital imaging of the murine pancreas
Wei Du, Christian Adkisson, Xianjun Ye, Camille L. Duran, Benson Chellakkan Selvanesan, Claudia Gravekamp, Maja H. Oktay, John C. McAuliffe, John S. Condeelis, Nicole C. Panarelli, Robert J. Norgard, Yogev Sela, Ben Z. Stanger, David Entenberg Open Biology.2022;[Epub] CrossRef - Intravital longitudinal cellular visualization of oral mucosa in a murine model based on rotatory side-view confocal endomicroscopy
Sujung Hong, Jingu Lee, Jieun Moon, Eunji Kong, Jehwi Jeon, Yeon soo Kim, Hyung-Ryong Kim, Pilhan Kim Biomedical Optics Express.2022; 13(8): 4160. CrossRef - Improved in vivo imaging method for individual islets across the mouse pancreas reveals a heterogeneous insulin secretion response to glucose
Henriette Frikke-Schmidt, Peter Arvan, Randy J. Seeley, Corentin Cras-Méneur Scientific Reports.2021;[Epub] CrossRef - The frontier of live tissue imaging across space and time
Qiang Huang, Aliesha Garrett, Shree Bose, Stephanie Blocker, Anne C. Rios, Hans Clevers, Xiling Shen Cell Stem Cell.2021; 28(4): 603. CrossRef - Intravital Laser-scanning Two-photon and Confocal Microscopy for Biomedical Research
Jieun Moon, Pilhan Kim Medical Lasers.2021; 10(1): 1. CrossRef - The Eye as a Transplantation Site to Monitor Pancreatic Islet Cell Plasticity
Erwin Ilegems, Per-Olof Berggren Frontiers in Endocrinology.2021;[Epub] CrossRef - Longitudinal Intravital Imaging of Tumor-Infiltrating Lymphocyte Motility in Breast Cancer Models
Inwon Park, Sujung Hong, Joon Seok, Stephani Edwina Lucia, Eunjoo Song, Mingyo Kim, Eunji Kong, Howon Seo, Yoonha Hwang, Soyeon Ahn, Seonghye Kim, Dong-Hyun Jang, Jae Hyuk Lee, Su-Hyung Park, Pilhan Kim, You Hwan Jo Journal of Breast Cancer.2021; 24(5): 463. CrossRef - Intravital longitudinal imaging of hepatic lipid droplet accumulation in a murine model for nonalcoholic fatty liver disease
Jieun Moon, Eunji Kong, Jingu Lee, Jinjoo Jung, Eunha Kim, Seung Bum Park, Pilhan Kim Biomedical Optics Express.2020; 11(9): 5132. CrossRef
|