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Yoonha Hwang  (Hwang Y) 1 Article
Basic Research
A Novel Pancreatic Imaging Window for Stabilized Longitudinal In Vivo Observation of Pancreatic Islets in Murine Model
Inwon Park, Sujung Hong, Yoonha Hwang, Pilhan Kim
Diabetes Metab J. 2020;44(1):193-198.   Published online May 29, 2019
DOI: https://doi.org/10.4093/dmj.2018.0268
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  • 13 Web of Science
  • 11 Crossref
AbstractAbstract PDFSupplementary MaterialPubReader   

Longitudinal imaging of murine pancreas is technically challenging due to the mechanical softness of the tissue influenced by peristalsis. Here, we report a novel pancreatic imaging window for long-term stabilized cellular-level observation of the islets in the pancreas in vivo. By spatially separating the pancreas from the bowel movement and physiologic respiration with a metal plate integrated in the imaging window, we successfully tracked the pancreatic islets up to three weeks and visualized the dumbbell-shape transformation from the single islet. This window can be a useful tool for long-term cellular-level visualization of the microstructure in the pancreas.

Citations

Citations to this article as recorded by  
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    Xuwen Peng, Yuke Wang, Jie Zhang, Zhihong Zhang, Shuhong Qi
    Frontiers in Immunology.2023;[Epub]     CrossRef
  • Minimizing Motion Artifacts in Intravital Microscopy Using the Sedative Effect of Dexmedetomidine
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    Microscopy and Microanalysis.2022; 28(5): 1679.     CrossRef
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    Open Biology.2022;[Epub]     CrossRef
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    Biomedical Optics Express.2022; 13(8): 4160.     CrossRef
  • Improved in vivo imaging method for individual islets across the mouse pancreas reveals a heterogeneous insulin secretion response to glucose
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    Scientific Reports.2021;[Epub]     CrossRef
  • The frontier of live tissue imaging across space and time
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    Cell Stem Cell.2021; 28(4): 603.     CrossRef
  • Intravital Laser-scanning Two-photon and Confocal Microscopy for Biomedical Research
    Jieun Moon, Pilhan Kim
    Medical Lasers.2021; 10(1): 1.     CrossRef
  • The Eye as a Transplantation Site to Monitor Pancreatic Islet Cell Plasticity
    Erwin Ilegems, Per-Olof Berggren
    Frontiers in Endocrinology.2021;[Epub]     CrossRef
  • Longitudinal Intravital Imaging of Tumor-Infiltrating Lymphocyte Motility in Breast Cancer Models
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    Journal of Breast Cancer.2021; 24(5): 463.     CrossRef
  • Intravital longitudinal imaging of hepatic lipid droplet accumulation in a murine model for nonalcoholic fatty liver disease
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    Biomedical Optics Express.2020; 11(9): 5132.     CrossRef

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