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Baddireddi Subhadra Lakshmi  (Lakshmi BS) 2 Articles
Response: An In Vitro Model to Probe the Regulation of Adipocyte Differentiation under Hyperglycemia (Diabetes Metab J 2013;37:176-80)
Kusampudi Shilpa, Thangaraj Dinesh, Baddireddi Subhadra Lakshmi
Diabetes Metab J. 2013;37(4):298-299.   Published online August 14, 2013
DOI: https://doi.org/10.4093/dmj.2013.37.4.298
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  • 28 Download
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An In Vitro Model to Probe the Regulation of Adipocyte Differentiation under Hyperglycemia
Kusampudi Shilpa, Thangaraj Dinesh, Baddireddi Subhadra Lakshmi
Diabetes Metab J. 2013;37(3):176-180.   Published online June 14, 2013
DOI: https://doi.org/10.4093/dmj.2013.37.3.176
  • 3,035 View
  • 34 Download
  • 8 Crossref
AbstractAbstract PDFPubReader   
Background

The aim of this study was an in vitro investigation of the effect of high glucose concentration on adipogenesis, as prolonged hyperglycemia alters adipocyte differentiation.

Methods

3T3-L1 preadipocytes differentiated in the presence of varying concentrations of glucose (25, 45, 65, 85, and 105 mM) were assessed for adipogenesis using AdipoRed (Lonza) assay. Cell viability and proliferation were measured using MTT reduction and [3H] thymidine incorporation assay. The extent of glucose uptake and glycogen synthesis were measured using radiolabelled 2-deoxy-D-[1-3H] glucose and [14C]-UDP-glucose. The gene level expression was evaluated using reverse transcription-polymerase chain reaction and protein expression was studied using Western blot analysis.

Results

Glucose at 105 mM concentration was observed to inhibit adipogenesis through inhibition of CCAAT-enhancer-binding proteins, sterol regulatory element-binding protein, peroxisome proliferator-activated receptor and adiponectin. High concentration of glucose induced stress by increasing levels of toll-like receptor 4, nuclear factor κB and tumor necrosis factor α thereby generating activated preadipocytes. These cells entered the state of hyperplasia through inhibition of p27 and proliferation was found to increase through activation of protein kinase B via phosphoinositide 3 kinase dependent pathway. This condition inhibited insulin signaling through decrease in insulin receptor β. Although the glucose transporter 4 (GLUT4) protein remained unaltered with the glycogen synthesis inhibited, the cells were found to exhibit an increase in glucose uptake via GLUT1.

Conclusion

Adipogenesis in the presence of 105 mM glucose leads to an uncontrolled proliferation of activated preadipocytes providing an insight towards understanding obesity.

Citations

Citations to this article as recorded by  
  • Adipogenesis-Related Metabolic Condition Affects Shear-Stressed Endothelial Cells Activity Responding to Titanium
    Thaís Silva Pinto, Anderson Moreira Gomes, Paula Bertin de Morais, Willian F. Zambuzzi
    Journal of Functional Biomaterials.2023; 14(3): 162.     CrossRef
  • Chronic and Transient Hyperglycemia Induces Changes in the Expression Patterns of IL6 and ADIPOQ Genes and Their Associated Epigenetic Modifications in Differentiating Human Visceral Adipocytes
    Adam Wróblewski, Justyna Strycharz, Ewa Świderska, Aneta Balcerczyk, Janusz Szemraj, Józef Drzewoski, Agnieszka Śliwińska
    International Journal of Molecular Sciences.2021; 22(13): 6964.     CrossRef
  • Effects of high glucose conditions on the expansion and differentiation capabilities of mesenchymal stromal cells derived from rat endosteal niche
    Ahmed Makki A. Al-Qarakhli, Norhayati Yusop, Rachel J. Waddington, Ryan Moseley
    BMC Molecular and Cell Biology.2019;[Epub]     CrossRef
  • Inhibition of WNT/β-catenin signaling under serum starvation and hypoxia induces adipocytic transdifferentiation in human leiomyoma cells
    Hiroshi Harada, Yojiro Tsuda, Kei Yabuki, Eisuke Shiba, Kazuyoshi Uchihashi, Atsuji Matsuyama, Yoshihisa Fujino, Toru Hachisuga, Masanori Hisaoka
    Laboratory Investigation.2018; 98(4): 439.     CrossRef
  • Effects of high glucose on caveolin-1 and insulin signaling in 3T3-L1 adipocytes
    Sara Palacios-Ortega, Maider Varela-Guruceaga, J. Alfredo Martínez, Carlos de Miguel, Fermín I. Milagro
    Adipocyte.2016; 5(1): 65.     CrossRef
  • Pathophysiological role of enhanced bone marrow adipogenesis in diabetic complications
    Meghan A Piccinin, Zia A Khan
    Adipocyte.2014; 3(4): 263.     CrossRef
  • Letter: AnIn VitroModel to Probe the Regulation of Adipocyte Differentiation under Hyperglycemia (Diabetes Metab J2013;37:176-80)
    In-Kyung Jeong
    Diabetes & Metabolism Journal.2013; 37(4): 296.     CrossRef
  • Response: AnIn VitroModel to Probe the Regulation of Adipocyte Differentiation under Hyperglycemia (Diabetes Metab J2013;37:176-80)
    Kusampudi Shilpa, Thangaraj Dinesh, Baddireddi Subhadra Lakshmi
    Diabetes & Metabolism Journal.2013; 37(4): 298.     CrossRef

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