- Transcription Factor Profile by Degenerate RT-PCR/SSCP: Application in 3T3-L1 Adipocyte Treated with TNF-alpha.
-
Yoo Lee Kim, Sang Hwa Lee, Young Kil Choi, Seo Yoon Chang, Yun Soo Kim, Soo Kyung Kim, Seok Won Park, Won Kun Park, Yong Wook Cho, Sang Jong Lee
-
Korean Diabetes J. 2007;31(5):410-420. Published online September 1, 2007
-
DOI: https://doi.org/10.4093/jkda.2007.31.5.410
-
-
 Abstract
 PDF
- BACKGROUND
Several high-throughput gene analysis techniques - differential display PCR, suppression subtraction hybridization (SSH), serial analysis of gene expression (SAGE), and DNA microarray - have permitted transcriptome profiling to understand the molecular pathogenesis of multifactorial diseases. But these techniques are of no great utility regarding feasibility, reproducibility, cost, and the amount of material required for analysis. To establish more practical method for transcription factor transcriptome profiling, we combined degenerate reverse transcriptase-polymerase chain reaction (RT-PCR) and single strand conformational polymorphism (SSCP) technique. METHODS: We categorized 417 human/mouse transcription factor mRNA into 92 small groups according to homology with ClustalW method and established 92 degenerate RT-PCR including common motives of the 92 small groups with the software program of CODEHOP, Primer Premier, Amplify 1.2. Further analysis on the amplified PCR products was performed by SSCP. This system was applied for the evaluation of changes on transcription factor transcriptome of differentiated 3T3-L1 adipocyte treated with TNF-alpha. RESULTS: 82 groups and 52 groups showed amplification of PCR before and after TNF-alpha treatment respectively and 24 groups showed significant amplification difference after TNF-alpha treatment. After TNF-alpha treatment for 48 hours, mRNA expressions of group 7, 30, and 33 which include adipocyte related transcription factors such as CEBP-alpha, RXR-alpha, PPAR-gamma were downregulated and mRNA expression of group 8 including preadipocyte abundant CEBP-beta was upregulated. These results are largely concordant with the results analyzed by oligonucleotide microarray. Randomly selected single PCR bands of group 28 and 75 on agarose electrophoresis displayed additional multiple bands by SSCP and necessitated addition of this technique to degenerate RT-PCR for further analysis. CONCLUSION: It could be suggested that degenerate RT-PCR/SSCP is practical method and could be used as a screening test for transcriptome profiling of various disease states with further validation study.
- Homeostasis Model Assessment In Korean Type 2 Diabetes Patients.
-
Hyun Ha Chang, Jin Woo Kim, Mee Suk Rhu, Cheol Young Park, Seung Joon Oh, Jeong Taek Woo, Sung Woon Kim, Young Seol Kim, Young Kil Choi
-
Korean Diabetes J. 2002;26(4):296-305. Published online August 1, 2002
-
-
-
Abstract
PDF
- BACKGROUND
Both insulin resistance and decreased insulin secretion have been known to be defects causing type 2 diabetes. However, decreased insulin secretion rather than insulin resistance has been suggested to be a more important factor in development of type 2 diabetes in Korea. Thus, we undertook this study to evaluate insulin resistance and beta cell function in Korean type 2 diabetes patients. METHODS: Retrospectively, we applied HOMA model to 1,233 type 2 diabetes pateints (575 males and 658 females) and assessed HOMA(beta cell) function and HOMA(IR). RESULTS: The HOMA(IR) of Korean male was 2.6 (0.05~39.53) and that of Korean female was 3.1 (0.04~53.54), and the HOMA(beta cell) of male was 22.3 (0.2~873.8)%, and that of female was 30.6 (0.37~1738.3)%. There was no significant difference. There was no significant correlation between HOMA(IR) and age and duration of diabetes, but there was significant correlation between HOMA(beta cell) and BMI, 0 min, 30 min insulin level and 0 min C-peptide level in oGTT. In the group of high HOMA(IR) than lower HOMA(IR), HOMA(beta cell) have more strong correlation with age at dignosis, BMI, duration of diabetes, FBS, 0 min and 60 min insulin, 0 min C-peptide and 24 hour urine C-peptide. CONCLUSION: In comparison with HOMA Model of San Antonio Heart Study, the HOMA(beta cell) and HOMA(IR) of Korean were lower, so the HOMA(beta cell) and HOMA(IR) may be different between ethnic groups. And further prospective analysis for the evaluation of insulin resistance and insulin secretion defect with HOMA model should be done in Korean type 2 diabeties.
- Gastric Dysfunction Assessed by Electrogastrography and the Acetaminophen Absorption Technique in Patients with Diabetic Neuropathy.
-
Hyun Ha Chang, Young Seol Kim, Sang Hwa Kim, Cheol Young Park, Seung Joon Oh, Jeong Taek Woo, In Myung Yang, Sung Woon Kim, Jin Woo Kim, Young Kil Choi
-
Korean Diabetes J. 2001;25(1):83-92. Published online February 1, 2001
-
-
-
Abstract
PDF
- BACKGROUND
In patients with diabetes mellitus, dysrhythmias of gastric myoelectrical activity are frequently associated with gastroparesis. Thus, we investigated the relationships between abnormal gastric myoelectrical activity and gastric emptying rate in patients with diabetes mellitus. METHODS: Using surface electrogastrography (Nippro, Tokyo, Japan) and acetaminophen absorption technique, we studied EGG and gastric emptying rate in 39 patients with diabetic neuropathy and 15 healthy volunteers as the control group. RESULTS: In two thirds of the patients with diabetes mellitus, fasting electrogastrography revealed instability of electrical control activity (ECA), as compared to 13% of the control. Postprandial amplitude and frequency increases in electrogastrography were observed in the control group. However, these postprandial responses were obtunded in patients with diabetes mellitus. In the assessment of gastric emptying rate, increases of plasma acetaminophen levels were delayed in obtunded postprandial responses of gastric myoelectrical activity. CONCLUSION: These results showed that the abnormal electrogastrography patterns were related to abnormal finding assessed acetaminophen absorption technique. Electrogastrography was thought to be a useful and non-invasive technique in assessing diabetic gastroparesis.
- Free Testosterone and Sex Hormone-Binding Globulin Level in Type 2 Diabetic Men.
-
Ki Deuk Nam, Young Seol Kim, Cheol Young Park, Seung Joon Oh, Deog Yoon Kim, Jeong Taek Woo, Sung Woon Kim, In Myung Yang, Jin Woo Kim, Young Kil Choi
-
Korean Diabetes J. 2000;24(6):699-707. Published online January 1, 2001
-
-
-
Abstract
PDF
- BACKGROUND
Insulin resistance is a risk factor for cardiovascular disease and type 2 diabetes mellitus. There are many previous studies indicating that insulin lowers serum sex hormone-binding globulin levels, and there is inverse correlation between insulin resistance and serum sex hormone-binding globulin levels in women. However, in men, a limited number of studies are available to explain the effect of sex hormone on age and insulin. Therefore, the present study was undertaken to investigate the relationship among free testosterone, hormone- binding globulin and age in type 2 diabetic men and control subjects. METHOD: Age, body mass index, total cholesterol, triglyceride, fasting blood sugar, and insulin concentrations were examined on 89 type 2 diabetic men and 47 control subjects. The free testosterone level was measured by commercially available double-antibody system (Radioimmunoassay). The sex hormone-binding globulin level was also measured by commercially available double-antibody system(Immunoradiometric assay). RESULTS: 1) Sex hormone-binding globulin level was significantly increased in patients with type 2 diabetes. However, there was no significant difference in free testosterone level between the two groups. 2) Sex hormone-binding globulin was positively correlated with age (r=0.4, p<0.001) in patients with type 2 diabetes. Sex hormone-binding globulin and free testos terone were not correlated with age in control sujects. 3) Free testosterone and sex hormone-binding globulin concentrations were not significantly related to serum insulin concentration after adjusting for age and body mass index. CONCLUSIONS: We observed increased sex hormone-binding globulin concentration in diabetes man, and was a positively related to age. Further studies are needed to understand the relationships between age, insulin resistance, testosterone, and sex hormone-binding globulin concentrations.
- Lowering Effect of Voglibose, Monotherapy on Uncontrolled Postprandial Glucose in Patients with Non-Insulin Dependent Diabetes Mellitus (NIDDM) Being Treated with Strict Diet Control: Multicenter Open-Study.
-
Jeong Taek Woo, Young Seol Kim, Young Kil Choi, Jin Woo Kim, In Myung Yang, Sung Woon Kim, Deog Yoon Kim, Kwang Won Kim, Moon Kyu Lee, Myung Shik Lee, Jae Hoon Jung, Kyu Jeong Ahn, Hyun Chul Lee, Young Deuk Song, Bong Soo Cha, Jee Hyun Lee, Hyung Joon Won
-
Korean Diabetes J. 1998;22(3):419-428. Published online January 1, 2001
-
-
-
Abstract
PDF
- BACKGROUND
It is sometimes very difficult to control the elevation of postprandial glucose with diet therapy only in patients with NIDDM partly because of their defective insulin response to glucose. Recently the alpha-glucosidase inhibitors which inhibit carbohydrate digestion and suppress or delay absorption of the final breakdown products, glucose and fructose when it is taken orally with meal have been widely used in the treatment of diabetes. The drugs, however, provoke the adverse effects e.g. flatulence, diarrhea etc. in some patients. Therefore we studied the efficacy of the more recently developed alpha glucosidase inhibitor, Voglibose (Basen, Cheiljedang) METHODS: Fifty five patients whose postprandial two hour serum glucose levels were more than 11.1 mmol/L despite the strict diet therapy during the 4 week observation period were assigned to receive Voglibose 0.2 mg before each meal t.i.d. for 8 weeks. Of 55 subjects, 41 were given Voglibose 0.3 mg t..i.d. for the last 4 weeks because of their poor glucose control, RESULTS: The postprandial one and two hour serum glucose levels significantly decreased after therapy; 1 hour: 17.5+4.4 mmol/L(prior to therapy), 15.4+3.8 mmol/L(4 week after), 14.8+5.1 mmol/L(8 week), p <0.00l, 2 hour: 16.7+4.5 mmol/L, 14.8+3.9 mmol/ L, 14.8+4.5 mmol/L, p<0.00 l, t-tests for paired samples. Total serum cholesterol and HDL cholesterol levels also significantly decreased(5.24+1.06 - 4.90+1.27 mmol/L, p=0.036, 1.34+0.66 1.16 +0.3l mmol/L, p=0.035 respectively) However, HbAlc, serum fructosamine, insulin and triglyceride levels were not significantly changed. The prevalence of the adverse effects due to Voglibose was 14%(10/71). All of them were less than grade II of WHO criteria and disappeared despite continuing therapy. CONCLUSION: Voglibose monotherapy is considered as having an glucose lowering effect in patients with NIDDM whose adequate postprandial blood glucose cannot be achieved with diet therapy only.
- Clinical effect of topical capsaicin in painful diabetic polyneuropathy.
-
Dai Ok Cho, Jae Man Kim, Deog Yoon Kim, Jeong Taek Woo, Sung Woon Kim, In Myung Yang, Jin Woo Kim, Young Seol Kim, Kwang Won Kim, Young Kil Choi
-
Korean Diabetes J. 1993;17(4):411-417. Published online January 1, 2001
-
-
-
Abstract
PDF
- No abstract available.
- The influence of feedback inhibition of insulin secretion by insulin itself in isolated rat islets.
-
Sung Hoon Kim, Sung Yi Kang, Deog Yoon Kim, Kwang Sik Seo, Jeong Taek Woo, Sung Woon Kim, In Myung Yang, jin Woo Kim, Young Seol Kim, Kwang Won Kim, Young Kil Choi
-
Korean Diabetes J. 1993;17(1):35-43. Published online January 1, 2001
-
-
-
Abstract
PDF
- No abstract available.
- Clinical study of cilostazol in diabetic patients with peripheralvascular disease.
-
Tai Hee Lee, Young Kil Choi, Kap Bum Huh, Soon Hyun Shin, Hong Kyu Lee, Ho Young Son, Young Seol Kim, Hyun Chul Lee, Min Young Chung
-
Korean Diabetes J. 1992;16(4):325-334. Published online January 1, 2001
-
-
-
Abstract
PDF
- No abstract available.
- Three dimensional long-term culture of rat islets using gelatin gel.
-
Deog Yoon Kim, Kwang Sik Seo, Eun Mi Koh, Jeong Taek Woo, Seong Woon Kim, In Myung Yang, Jin Woo Kim, Young Seol Kim, Kwang Won Kim, Young Kil Choi
-
Korean Diabetes J. 1992;16(4):299-307. Published online January 1, 2001
-
-
-
Abstract
PDF
- No abstract available.
- Serum lipoprotein(a) concentration in patients with diabetic retinopathy.
-
Seung Joon Oh, Deog Yoon Kim, Jung Taek Woo, Sung Woon Kim, In Myung Yang, Ji Woo Kim, Young Seol Kim, Kwang Won Kim, Young Kil Choi
-
Korean Diabetes J. 1992;16(4):281-287. Published online January 1, 2001
-
-
-
Abstract
PDF
- No abstract available.
- Combined effect of hyperglycemia and interleukin-1 beta on the insulin secretion of isolated rat islets.
-
Kwang Won Kim, Deog Yoon Kim, Kwang Sik Seo, Eun Mi Koh, Jeong Taek Woo, Sung Woon Kim, In Myung Yang, Jin Woo Kim, Young Seol Kim, Young Kil Choi
-
Korean Diabetes J. 1992;16(3):187-198. Published online January 1, 2001
-
-
-
Abstract
PDF
- No abstract available.
- Increased aldose reductase activity of cultured endothelial cell inhigh glucose media and reserve by epalrestat.
-
Seug Young Na, Jung Taik Woo, Jin Woo Kim, Kwang Won Kim, Young Seol Kim, Young Kil Choi, Kang Sik Seo, Jeong Rung Paeng
-
Korean Diabetes J. 1992;16(1):17-23. Published online January 1, 2001
-
-
-
Abstract
PDF
- No abstract available.
- Changes of plasma endothelin in non-insulin dependent diabetes mellitus.
-
Jeung Taek Woo, Hae Ran Lee, Jin Woo Kim, Young Seol Kim, Kwang Won Kim, Young Kil Choi
-
Korean Diabetes J. 1991;15(2):251-256. Published online January 1, 2001
-
-
-
Abstract
PDF
- No abstract available.
|
KDA
