- High Glucose and/or Free Fatty Acid Damage Vascular Endothelial Cells via Stimulating of NAD(P)H Oxidase-induced Superoxide Production from Neutrophils.
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Sang Soo Kim, Sun Young Kim, Soo Hyung Lee, Yang Ho Kang, In Ju Kim, Yong Ki Kim, Seok Man Son
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Korean Diabetes J. 2009;33(2):94-104. Published online April 1, 2009
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DOI: https://doi.org/10.4093/kdj.2009.33.2.94
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- BACKGROUND
Oxidative stress and inflammation are important factors in the pathogenesis of diabetes and contribute to the development of diabetic complications. To understand the mechanisms that cause vascular complications in diabetes, we examined the effects of high glucose and/or free fatty acids on the production of superoxide from neutrophils and their role in endothelial cell damage. METHODS: Human neutrophils were incubated in the media containing 5.5 mM D-glucose, 30 mM D-glucose, 3 nM oleic acid, or 30 microM oleic acid for 1 hour to evaluate superoxide production through NAD(P)H oxidase activation. Human aortic endothelial cells were co-cultured with neutrophils exposed to high glucose and oleic acid. We then measured neutrophil adhesion to endothelial cells, neutrophil activation and superoxide production, neutrophil-mediated endothelial cell cytotoxicity and subunits of neutrophil NAD(P)H oxidase. RESULTS: After 1 hour of incubation with various concentrations of glucose and oleic acid, neutrophil adherence to high glucose and oleic acid-treated endothelial cells was significantly increased compared with adhesion to low glucose and oleic acid-treated endothelial cells. Incubation of neutrophils with glucose and free fatty acids increased superoxide production in a dose-dependent manner. High glucose and oleic acid treatment significantly increased expression of the membrane components of NAD(P)H oxidase of neutrophil (gp91(phox)). Endothelial cells co-cultured with neutrophils exposed to high glucose and oleic acid showed increased cytolysis, which could be prevented by an antioxidant, N-acetylcysteine. CONCLUSION: These results suggest that high glucose and/orfree fatty acidsincrease injury of endothelial cells via stimulating NAD(P)H oxidase-induced superoxide production from neutrophils.
- Association of Serum Cystatin C with Metabolic Syndrome and Its Related Components in Korean Adults.
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Sun Young Kim, Sang Heon Song, Yun Kyung Jeon, Ji Ryang Kim, Jung Ho Bae, Sang Soo Kim, Soo Hyung Lee, Seok Man Son, In Ju Kim, Yong Ki Kim, Yang Ho Kang
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Korean Diabetes J. 2008;32(5):409-417. Published online October 1, 2008
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DOI: https://doi.org/10.4093/kdj.2008.32.5.409
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2,924
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Abstract
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- BACKGROUND
Serum cystatin C has been reported as a better marker than serum creatinine for estimation of kidney function and may be associated with cardiovascular disease. The aim of this study was to elucidate the association of serum cystatin C with metabolic syndrome (MS), a constellation of cardiovascular risk factors, and its related components and the usefulness of serum cystatin C for the cardiovascular risk assessment. METHODS: 1,468 healthy subjects (814 men and 655 women), who visited health promotion center of Pusan National University Hospital for routine medical checkup were included. MS was defined by modified, revised National Cholesterol Education Program (NCEP) Adult Treatment Panel (ATP) III criteria. RESULTS: Mean serum cystatin C value was 0.87 +/- 0.17 mg/L. In partial correlation analysis adjusted by age, sex and Glomerular Filtration Rate (GFR), cystatin C was associated with most of metabolic parameters and especially had significant positive correlation with waist circumference (r = 0.215), triglyceride (TG) (r = 0.141), diastolic blood pressure (BP) (r = 0.116), and correlated negatively with high density lipoprotein (HDL) cholesterol (r = -0.152) (all P < 0.001). There were increasing trends of prevalence of MS with the increase of quartiles of cystatin C and as the number of MS components increased, cystatin C values significantly increased. Serum cystatin C was also significantly increased in MS (0.90 +/- 0.19 mg/L vs. 0.86 +/- 0.16 mg/L). In stepwise multiple regression analysis including the components of MS, Waist circumference, diastolic BP, triglyceride, and HDL cholesterol were independent determinants of serum cystatin C, but with creatinine, only waist circumference was independent determinant. CONCLUSIONS: Serum cystatin C was closely associated with MS and its related cardiovascular risk factors and might be useful as a tool of cardiovascular risk assessment.
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Citations
Citations to this article as recorded by  - Cystatin C in Patients of Metabolic Syndrome and its Correlation with the Individual Components of Metabolic Syndrome
Sunita Aghade, Jayshree S Bavikar, Pragati S Kadam, Reshakiran J Shendye
Indian Journal of Medical Biochemistry.2019; 23(2): 293. CrossRef - Cystatin C as a Predictor for Diabetes according to Glycosylated Hemoglobin Levels in Korean Patients
Eon Ju Jeon, Ji Hyun Lee
Diabetes & Metabolism Journal.2016; 40(1): 32. CrossRef - Association of Obesity with Serum Cystatin C in Korean Adults
Yang Ho Kang
The Korean Journal of Obesity.2015; 24(4): 199. CrossRef
- Oxidative Stress and Cell Dysfunction in Diabetes: Role of ROS Produced by Mitochondria and NAD(P)H Oxidase.
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Sang Soo Kim, Seok Man Son
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Korean Diabetes J. 2008;32(5):389-398. Published online October 1, 2008
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DOI: https://doi.org/10.4093/kdj.2008.32.5.389
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2,741
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76
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- Oxidative stress has been considered to be a major contributor to the pathogenesis of the diabetic macrovascular and microvascular complications. In the absence of an appropriate antioxidant defense mechanism, increased oxidative stress leads to the activation of stress-sensitive intracellular signaling pathways and the formation of gene products that cause damage and contribute to the late complications ofdiabetes. The source of reactive oxygen species (ROS) in the pancreatic beta cells and insulin sensitive cells has postulated to be the mitochondrial electron transport chain. NAD(P)H oxidase-dependent ROS production is also important as the source both in pancreatic beta cells and other cells. NAD(P)H oxidase mediated ROS can alter parameters of signal transduction, insulin secretion, insulin action, cell proliferation and cell death. Additionally, oxidative stress as the pathogenic mechanism linking insulin resistance with dysfunction of both pancreatic beta cells and endothelial cells, eventually leads to diabetes and its complications. Further investigation of the mechanisms and its therapeutic interventions based on focusing NAD(P)H oxidase associated ROS production in the islet cells and other islet cells are needed
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Citations
Citations to this article as recorded by - Cytoprotective Effect of Hot-Water Extracts of Protaetia brevitarsis seulensis Larvae in Cisplatin-Treated Macrophages
Bo-Gyeong Yoo, Jun-Pyo Hong, Ha-Yeon Song, Eui-Hong Byun
Journal of the Korean Society of Food Science and Nutrition.2022; 51(12): 1252. CrossRef - The Effects of LR3 and SP6 Acupuncture on Renal Damage in Streptozotocin-induced Diabetic Mice
Cho In Lee, Hyun Jong Lee, Yun Kyu Lee, Seong Chul Lim, Jae Soo Kim
The Acupuncture.2015; 32(3): 41. CrossRef - Effect of Chungkukjang supplementation on oxidative stress and antioxidant nutrients of diabetic rats induced by streptozotocin
Heyun-Sook Park, Hyun-Joo Kong, Eon-Hee Lee, Eun-Mi Choi, Joung-Hyeon Jang, Myoung-Hee Lee, Ju-Yeon Hong, Su-Jung Hwang, Hyeon-A Jung, Kyung-Mi Yang
Korean Journal of Food Preservation.2015; 22(2): 281. CrossRef - The Effects of Several Halophytes on Insulin Resistance in Otsuka Long-evans Tokushima Fatty Rats
Jeong-Yong Cho, Zhangjun Huang, Sun-Young Park, Kyung-Hee Park, Tong-Kun Pai, So-Young Kim, Haeng-Ran Kim, Kyung-Sik Ham
Korean Journal of Food Science and Technology.2014; 46(1): 100. CrossRef - Protective Effects of Radiation-induced Blackberry Mutant Extract on Carbon Tetrachloride (CCl4)-induced Liver Injury in Sprague-Dawley Rats
Byoung Ok Cho, Chang-Wook Lee, Yangkang So, Chang-Hyun Jin, Hong-Sun Yook, Myung-Woo Byun, Yong-Wook Jeong, Jong Chun Park, Il-Yun Jeong
Journal of the Korean Society of Food Science and Nutrition.2014; 43(6): 807. CrossRef - Antioxidant Activities and α-Glucosidase Inhibitory Effect of Water Extracts from Medicinal Plants
Hyun Sook Kim, Tae Woo Kim, Dae Jung Kim, Jae Sung Lee, Kyoung Kon Kim, Myeon Choe
Korean Journal of Medicinal Crop Science.2013; 21(3): 197. CrossRef - Antioxidant Effect of Hot water and Ethanol extracts from Cheonnyuncho (Opuntia humifusa) on Reactive Oxygen Species (ROS) Production in 3T3-L1 Adipocytes
Bo-Ra Yoon, Young-Jun Lee, Sun-Gu Kim, Jung-Young Jang, Hyo-Ku Lee, Seong-Kap Rhee, Hee-Do Hong, Hyeon-Son Choi, Boo-Yong Lee, Ok-Hwan Lee
Korean Journal of Food Preservation.2012; 19(3): 443. CrossRef - Inhibitory Effects of Panax ginseng C. A. Mayer Treated with High Temperature and High Pressure on Oxidative Stress
Bo-Ra Yoon, Young-Jun Lee, Hee-Do Hong, Young-Chul Lee, Young-Chan Kim, Young Kyoung Rhee, Kyung-Tack Kim, Ok-Hwan Lee
The Korean Journal of Food And Nutrition.2012; 25(4): 800. CrossRef - Effect of Dietary Supplementation of β-Carotene on Hepatic Antioxidant Enzyme Activities and Glutathione Concentration in Diabetic Rats
Jung-Hyun Jang, Kyeung-Soon Lee, Jung-Sook Seo
Journal of the Korean Society of Food Science and Nutrition.2011; 40(8): 1092. CrossRef - An Association between 609 C →T Polymorphism in NAD(P)H: Quinone Oxidoreductase 1 (NQO1) Gene and Blood Glucose Levels in Korean Population
Dohee Kim
Korean Diabetes Journal.2009; 33(1): 24. CrossRef
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